A set of vertices S is a determining set of a graph G if every automorphism of G is uniquely determined by its action on S. The determining number of G is the minimum cardinality of a determining set of G. This paper studies the determining number of Kneser graphs. First, we compute the determining number of a wide range of Kneser graphs, concretely K_n:k with n≥k(k+1) / 2+1. In the language of group theory, these computations provide exact values for the base size of the symmetric group S_n acting on the k-subsets of {1,…, n}. Then, we establish for which Kneser graphs K_n:k the determining number is equal to n-k, answering a question posed by Boutin. Finally, we find all Kneser graphs with fixed determining number 5, extending the study developed by Boutin for determining number 2, 3 or 4.